Product

Agilent liquid chromatography system

The new Agilent 1260 Infinity series offers unlimited possibilities. It provides a complete solution for liquid chromatography, providing you with chromatographic performance within your budget. No matter what application requirements you have, whether now or in the future, the universal technology in this series of products will help you improve laboratory efficiency and reduce work costs. Because it comes from Agilent and has over 40 years of technological innovation experience in chromatography, it definitely lives up to expectations.

Product details

Agilent liquid chromatography system

Agilent 1290 InfinityLC liquid chromatography has ended the debate on UHPLC. Now, we have raised the standards of HPLC, and the new Agilent 1220 and Agilent 1260 InfinityLC allow you to obtain the performance of RRLC at the price of HPLC: a system pressure resistance of 600bar and a detector speed of 80Hz. Moreover, all systems have a sensitivity increase of up to 10 times and are compatible with all your HPLC methods, ensuring no risk of replacing existing instruments.

Along with providing these new systems, there are always applicable HPLC, RRLC, and UHPLC solutions for various applications and budget requirements. Add your trusted high-quality brand name - Agilent.

·The working principle of the pump: low-pressure quaternary mixing, integrated with high-performance degassing unit

·Flow rate range: 0.05-5mL/min at 600bar; 5-10mL/min at 200bar

·Maximum pressure: 600bar

·Injection principle: Flow design, no overflow (extraction volume equals injection volume). Can sample bottles be used? Automatic sampler and sample bottle, as well as micro dropper tray automatic sampler

·Injection volume range: 0.1-100 μ L, increment of 0.1 μ L, larger injection volume can be selected

·Column specifications: Length: 300mm, Inner diameter: 0.05 × 8mm

·Temperature range: 10 ° C to 80 ° C below room temperature; maximum temperature up to 100 ° C with 1290 Infinity column temperature box option

·Temperature stability:<± 0.15 ° C; with 1290Infinity column temperature box option,<± 0.05 ° C

·Detector data acquisition rate: UV detector 80Hz; fluorescence detector 74Hz; evaporative light scattering detector 60Hz; 1290Infinity detector has a higher data acquisition rate

·UV noise level: as low as 0.6uAU/cm (1260InfinityDD, 60mm flow cell)

·Maintenance and system testing: Open the front panel to maintain all components; The maintenance guide is on the multimedia CD-ROM; Full system testing time (OQ/PV)<4 hours

·Standard GLP features: Early maintenance feedback - EMF (tracking lamp usage time, usage status, injection frequency, electronic record limitations, and error messages)

·Extended GLP features: Data Recovery Card (DRC) for data security (preventing data loss due to communication interruptions - depending on the actual detector selected)

·(For specific systems, please refer to the performance indicators of individual modules): Radio Frequency Identification (RFID), used for tracking data. The RFID tags of the circulation pool and UV lamp can clearly track the measurement conditions

·System control: Controlled through local workstation software, client/server network data system, and/or local handheld control module (1200Infinity series handheld controller). Default control: LAN/CAN

Agilent 1260 Infinity Quaternion Pump and Agilent 1260 Infinity VL Quaternion Pump

1. Traffic volume

Flow accuracy: ≤ 0.07% RSD or 0.02minSD, whichever is greater (calculated based on retention time at constant room temperature)

Flow accuracy: ± 1% or 10 μ L/min, whichever is higher (100bar, water flow rate)

Range: Set value from 0.001 to 10.0mL/min, increment 0.001ml/min

2. Pressure

Agilent 1290 InfinityLC liquid chromatography working range:

1) 1260Infinity Four Element Pump

·60MPa (600bar, 8700psi) maximum 5ml/min

·0-20MPa (0-200bar, 0-2950psi) maximum height of 10ml/min

2) 1260InfinityVL Four Element Pump:

·40MPa (400bar, 5880psi) maximum 5ml/min

·0-20MPa (0-200bar, 0-2950psi) maximum height of 10ml/min

Pulsation: When the flow rate of isopropanol is 1mL/min, under all pressures>1MPa (>10bar,>147psi), the amplification is<2% (generally<1.3%)... Required value TBD

Compressibility compensation: Users can choose based on the compressibility setting of the mobile phase

3. Gradient

Delay volume: 1260InfinityVL quaternary pump: 800-1100 μ L, depending on back pressure

Mixing accuracy:<0.20% SD at 0.2mL/min and 1mL/min

4. PH range

Recommended range: 1.0-12.5; When pH<2.3, the solvent should not contain acid, otherwise it will damage the stainless steel

5. Degassing

Integrated degassing element: 4 channels, maximum height of 10ml/min

Performance indicators of Agilent 1260 Infinity standard automatic sampler

Effective when equipped with a 100 μ L standard measuring head

1. Injection volume range:

·0.1-100 μ L, with increments of 0.1 μ L

·Up to 1500 μ L when equipped with multiple extraction upgrade toolkits (requiring hardware modifications)

2. Accuracy: within 5-100 μ L,<0.25% RSD, within 1-5 μ L,<1%, variable volume

3. Sample viscosity range: 0.2~5cp

4. Sample capacity:

·100 × 2ml sample bottle on 1 sample tray

·40 × 2ml sample bottle on 1/2 plate

·15 × 6ml on 1/2 plate (limited to Agilent sample bottles only)

5. Injection cycle time: usually 50 seconds, depending on the extraction speed and injection volume

6. Cross contamination: generally<0.1%,<0.05% when cleaning with external needles

7. Operating pressure range: up to 600bar (60MPa or 8702psi)

Performance indicators:

Effective when equipped with a 900 μ L standard measuring head

1. Injection volume range:

·0.1-900 μ L, with increments of 0.1 μ L (recommended increment is 1 μ L)

·When using the multiple extraction upgrade toolkit (requiring hardware modifications), the maximum output is up to 1800 μ L

2. Accuracy:

·Within 5-2000 μ L, the peak area accuracy is generally less than 0.5% RSD

·Within 2000-5000 μ L, the peak area accuracy is generally less than 1% RSD

·Within 1-5 μ L, the peak area accuracy is generally less than 3% RSD

·Variable volume

3. Sample viscosity range: 0.2~5cp

4. Sample capacity:

·On one sample tray, a 100 × 2ml sample bottle is placed,

·40 × 2ml sample bottle on 1/2 plate

·15 × 6ml on 1/2 plate (limited to Agilent sample bottles only)

5. Injection cycle time: usually 50 seconds, depending on the extraction speed and injection volume

6. Cross contamination: generally<0.1%,<0.05% when cleaning with external needles

7. Operating pressure range: up to 200 bar (20MPa or 2901psi)

Performance indicators of 1260Infinity high-performance automatic sampler

1. Communication: Local Area Network (CAN). RS232C, APG remote standard,? Optional four external contact closures and BCD digital output

2. Safety functions: leakage detection, safety leakage treatment, and maintenance; Regional low voltage, error detection and display

3. Injection volume range:

·0.1-100 μ L, with increments of 0.1 μ L

·Up to 1500 μ L with multiple extraction components (requiring hardware modifications)

4. Injection accuracy:

·Within 5-40 μ L,<0.25%

·Within 2-5 μ L,<0.5%

·Within 1-2 μ L,<0.7%

·<1.5% within 0.5~1 μ L,

5. Injection accuracy: ± 1% (10 μ L, n=10)

6. Sample viscosity range: 0.2~5cp

7. Sample capacity:

·2 porous plates (MTP)+10 × 2ml sample bottles

·108 × 2mL sample bottles on a 2 × 54 sample bottle tray, plus 10 additional 2mL sample bottles

·30 × 6mL sample bottles on a 2x15 sample bottle tray, plus 10 additional 2mL sample bottles

·54 Eppendorf tubes (0.5/1.5/2.0 mL) on a 2 × 27 Eppendorf tube sample tray

·Also compatible with Agilent 1200 series sample capacity expansion to further expand sample capacity

8. Injection cycle time: Normally<30s, use the following standard conditions:

·Default extraction speed: 200 μ L/min

·Default export speed: 200 μ L/min

·Injection volume: 5 μ L

9. Cross contamination routine<0.004% or 40ppm, using the following conditions:

·Chromatography column: 2.1x50mm ZORBAXSSBC18,1.8 μ m

·Sample: Chlorhexidine dissolved in water+0.1% TFA

·Mobile phase: water+0.05% TFA, acetonitrile+90.045% TFA

·Flow rate: 0.5mL/min

·Gradient: 0min 30% B, 3min 60% B?

·Injection volume: 1 μ L external needle washed with water+0.1% TFA, extraction and extraction speed=100 μ L/min

·Column temperature: 50 ° C

·MSDAPI-ES anode: SIM ion 505.30

·Fragmentation voltage: 70

·Gas temperature: 250 ° C

·Dry gas flow rate: 12L/min

·Vcap：3000V

10. GLP features: Early maintenance feedback (EMF), electronic recording of maintenance and error information.

Agilent 1260 Infinity Column Temperature Box

1. Temperature range: from 10 ° C below room temperature to 80 ° C

2. Temperature stability: ± 0.15 ° C

3. Temperature control accuracy: ± 0.8 ° C, calibrated ± 0.5 ° C

4. Column capacity: Three 30cm chromatography columns

5. Heating/; Time to cool down to the set temperature

·From room temperature to 40 ° C, 5 minutes are required

·It takes 10 minutes to go from 40 ° C to 20 ° C

6. Internal volume: 3 μ L left heat exchanger; Right heat exchanger 6 μ L

7. GLP: Column Identification Module, used for GLP to record column types

The performance indicators are effective for distilled water at room temperature (25 ° C), with a temperature setting of 40 ° C and a flow rate range of 0.1 to 5.0 mL/min.

Agilent 1260 Infinity Variable Wavelength Detector (G1314F)

1. Detector type: Dual beam photometer

2. Light source: deuterium lamp

3. Number of signals: 1

4. Maximum data acquisition rate: 80Hz

5. Noise: ± 0.25x10-5AU, at 230nm *

6. Drift: at 230nm, 1x10-4AU/hour

7. Linear: upper limit>2.5AU

8. Wavelength range: 190~600nm

9. Wavelength accuracy: ± 1nm, automatically calibrated with deuterium lamp, verified with holmium oxide filter

10. Slot width: Within the entire wavelength range, generally 6.5nm

11. Time programmed: wavelength, polarity, peak width, light on/off

12. Flow pool

·Standard type: volume 14 μ l, optical path 10mm, maximum pressure 40bar (580psi)

·High pressure type (for SFC): volume 14 μ l, optical path 10mm, maximum pressure 400bar (5802psi)

·Micro type: volume 2 μ l, optical path 3mm, maximum pressure 120bar (1741psi)

·Semi micro type: volume 5 μ L, optical path 6mm, maximum pressure 40bar (580psi)

13. Spectral tool: stop flow spectral scanning

14. Analog output: Recorder/integrator, 100mV or 1V, 1 signal output

15. Communication

·LAN, Controller Area Network (CAN), RS-232C, APG Remote Control: Ready

·Start, Stop, and Close

·Signal

16. GLP feature: Early Maintenance Feedback (EMF) continuously records instrument usage, such as lamp usage time and user set deadlines, and provides feedback information. Electronic recording of maintenance and errors. RFID is used to electronically record the status of flow cells and UV sensors, including optical path length, volume, product model, serial number, test pass, and usage status. Verify wavelength accuracy using the built-in holmium oxide filter

17. Security and Maintenance: Comprehensive diagnosis, error detection, and display are carried out through Agilent handheld controllers and Agilent online laboratory consultant software. Leak detection, safe leak handling, and leak output signal (used to shut down the pump system). The voltage in the main maintenance area is relatively low

18. Other: Electronic Temperature Control (ETC), used for the entire optical component

*Under specific conditions

Agilent 1260 Infinity Variable Wavelength Detector (G1314B)

1. Detector type: Dual beam photometer

2. Light source: deuterium lamp

3. Number of signals: 1

4. Maximum acquisition rate: 13Hz

4. Noise: ± 0.5x10-5AU, at 230nm *

5. Drift: at 230nm, 3x10-4AU/hour

6. Linear: upper limit>2.0AU

7. Wavelength range: 190~600nm

8. Wavelength accuracy: ± 1nm, automatically calibrated with deuterium lamp, verified with holmium oxide filter

9. Slot width: Within the entire wavelength range, generally 6.5nm

10. Program wavelength, polarity, peak width, and light on/off according to time

11. Flow pool

·Standard Type: Volume 14 μ l, Optical Path 10mm, Maximum Pressure 40bar (580psi)

·High pressure type (for SFC): volume 14 μ l, optical path 10mm, maximum pressure 400bar (5802psi)

·Micro type: volume 2 μ l, optical path 3mm, maximum pressure 120bar (1741psi)

·Semi micro type: volume 5 μ L, optical path 6mm, maximum pressure 40bar (580psi)

12. Spectral tool: stop flow spectral scanning

13. Analog output: Recorder/integrator, 100mV or 1V, 1 signal output

14. Communication

·LAN, Controller Area Network (CAN), RS-232C, APG Remote Control: Ready

·Start, Stop, and Close

·Signal

15. GLP feature: Early Maintenance Feedback (EMF) continuously records instrument usage, such as lamp usage time and user set deadlines, and provides feedback information. Electronic recording of maintenance and errors. Verify wavelength accuracy using the built-in holmium oxide filter

16. Security and maintenance are comprehensively diagnosed, error detected, and displayed through Agilent handheld controllers and Agilent online laboratory consultant software. Leak detection, safe leak handling, and leak output signal (used to shut down the pump system). The voltage in the main maintenance area is relatively low

Performance indicators: Agilent 1260 InfinityVLPlus variable wavelength detector (G1314C)

1. Detector type: Dual beam photometer

2. Light source: deuterium lamp

3. Number of signals: 1

4. Maximum data acquisition rate: 55Hz

5. Noise: At 230nm *,<± 0.5x10-5AU

6. Drift:<3x10-4AU/hour at 230nm

7. Linear: upper limit>2.0AU

8. Wavelength range: 190~600nm

9. Wavelength accuracy: ± 1nm, automatically calibrated with deuterium lamp, verified with holmium oxide filter

10. Slot width: Within the entire wavelength range, generally 6.5nm

11. Time programmed: wavelength, polarity, peak width, light on/off

12. Flow pool

·Standard type: volume 14 μ l, optical path 10mm, maximum pressure 40bar (580psi)

·High pressure type (for SFC): volume 14 μ l, optical path 10mm, maximum pressure 400bar (5802psi)

·Micro type: volume 2 μ l, optical path 3mm, maximum pressure 120bar (1741psi)

·Semi micro type: volume 5 μ L, optical path 6mm, maximum pressure 40bar (580psi)

13. Spectral tool: stop flow spectral scanning

14. Analog output: Recorder/integrator, 100mV or 1V, 1 signal output

15. Communication

·LAN, Controller Area Network (CAN)

·RS-232C, APG remote control: ready, start, stop, and close signals

Performance indicators: 1260 Infinity multi wavelength detector (G1365C)

1. Detector type: 1024 diode elements

2. Light source: deuterium lamp and tungsten lamp

3. Number of signals: 8

4. Maximum sampling rate: 80Hz

5. Short term noise: at a wavelength of 254/4nm, ± 0.6x10-5AU; At a wavelength of 750nm, TC2 seconds

6. Drift:<0.9x10-3AU/hour at 254nm

7. Linear: at 265nm,>2.0AU (5%)

8. Wavelength range: 190~950nm

9. Wavelength accuracy: ± 1nm, automatically calibrated using a deuterium lamp; Validation using holmium oxide filter

10. Slot width: programmable: 1,2,4,8,16nm

11. Diode width:<1nm

12. Wavelength focusing: programmable, 1-400nm, increments of 1nm

13. Flow pool

·Standard type: volume 13 μ L, optical path 10mm flow cell, maximum pressure resistance 120bar (1740psi)

·Semi micro: volume 5 μ L, optical path 6mm, maximum pressure resistance 120bar (1740psi)

·Trace: Volume 2 μ L, optical path 3mm, maximum pressure resistance 120bar (1740psi)

·Half nanoliter: 500 nanoliters, optical path length of 10mm, maximum pressure resistance of 50bar (725psi)

·Nano liter type: 80 nanoliters, optical path length 6mm, maximum pressure resistance 50bar (725psi)

·High voltage type (for SFC): volume 1.7 μ L, optical path 6mm, maximum withstand voltage 400bar (5802psi)

·3mm preparation type: 3mm optical path, maximum pressure resistance of 120bar (1740psi)

·0.3mm preparation type: 0.3mm optical path, maximum pressure resistance of 20bar (291psi), quartz

·0.06mm preparation type: 0.06mm optical path length, maximum pressure resistance of 20bar (291psi), quartz

14. Time programmed: wavelength, polarity, peak width, lamp bandwidth, auto balance, wavelength range, threshold, spectral storage mode, analog output recorder/integrator: 100mV or 1V, 2 signal outputs

15. Communication

·LAN, Controller Area Network (CAN),

·RS-232C, APG remote control: ready

·Start, stop, and stop signals

16. GLP characteristics:

·Data recovery card to prevent data loss. RFID is used for electronic recording of the status of flow tanks and UV sensors, including optical path length, volume, product model, serial number, test pass, and usage status

·Early Maintenance Feedback (EMF) continuously records instrument usage, such as lamp usage time and user set deadlines, and provides feedback information. Electronic recording of maintenance and errors. Verification of wavelength accuracy using built-in holmium oxide filter

18. Other: Electronic Temperature Control (ETC) for all optical components

Performance indicators: 1260InfinityVL multi wavelength detector (G1365D)

1. Detector type: 1024 diode elements

2. Light source: deuterium lamp and tungsten lamp

3. Number of signals: 8

4. Maximum sampling rate: 20Hz

5. Short term noise: at a wavelength of 254/4nm, ± 0.6x10-5AU; At a wavelength of 750nm, TC2 seconds

6. Drift:<0.9x10-3AU/hour at 254nm

7. Linear: at 265nm,>2.0AU (5%)

8. Wavelength range: 190~950nm

9. Wavelength accuracy: ± 1nm, automatically calibrated using a deuterium lamp; Verify with holmium oxide filter

10. Slot width: programmable: 1,2,4,8,16nm

11. Diode width:<1nm

12. Wavelength focusing: programmable, 1-400nm, increments of 1nm

13. Flow pool

·Standard type: volume 13 μ L, optical path 10mm flow cell, maximum pressure resistance 120bar (1740psi)

·Semi micro: volume 5 μ L, optical path 6mm, maximum pressure resistance 120bar (1740psi)

·Trace: volume 2 μ L, optical path 3mm, maximum pressure resistance 120bar (1740psi)

·Half nanoliter: 500 nanoliters, optical path length 10mm, maximum pressure resistance 50bar (725psi)

·Nano liter type: 80 nanoliters, optical path length 6mm, maximum pressure resistance 50bar (725psi)

·High voltage type (for SFC): volume 1.7 μ L, optical path 6mm, maximum withstand voltage 400bar (5802psi)

·3mm preparation type: 3mm optical path, maximum pressure resistance 120bar (1740psi)

·0.3mm preparation type: 0.3mm optical path, maximum pressure resistance of 20bar (291psi), quartz

·0.06mm preparation type: 0.06mm optical path length, maximum pressure resistance of 20bar (291psi), quartz

14. Time programmed: wavelength, polarity, peak width, lamp bandwidth, auto balance, wavelength range, threshold, spectral storage mode

15. Analog output: Recorder/integrator: 100mV or 1V, 2 signal outputs

16. Communication

·LAN, Controller Area Network (CAN)

·RS-232C, APG remote control: ready

·Start, stop, and stop signals

17. GLP characteristics:

·RFID is used for electronic recording of the status of flow tanks and UV sensors, including optical path length, volume, product model, serial number, test pass, and usage status

18. Security and Maintenance: Comprehensive diagnosis, error detection, and display are carried out through Agilent handheld controllers and Agilent online laboratory consultant software. Leak detection, safe leak handling, and leak output signal (used to shut down the pump system). The voltage in the main maintenance area is relatively low

19. Other: Electronic Temperature Control (ETC) for all optical components

Performance indicators: 1260 Infinity diode array detector (G4212B)

1. Detector type: 1024 diode elements

2. Light source: deuterium lamp

3. Number of signals: 8

4. Maximum sampling rate: 80Hz (spectrum and signal)

5. Short term noise

·At 230/4nm,<± 3x10-6AU, slit width 4nm, TC2 seconds, ASTM， Equipped with 10mm maximum brightness card sleeve circulation pool

·At 230/4nm, generally<± 0.6x10-6AU/cm, slit width 4nm, TC2 seconds, ASTM with 60mm maximum light intensity card sleeve flow cell

6. Drift:<0.5x10-3AU/hour at 230nm

7. Linear: at 265nm,>2.0AU (5%); Generally 2.5AU (5%)

8. Wavelength range: 190~640nm

9. Wavelength accuracy: ± 1nm, deuterium line lamp automatically calibrated

10. Slot width: 4nm (fixed)

11. Diode width: approximately 0.5nm

12. Wavelength focusing: programmable, 1-400nm, increments of 1nm

13. Flow pool

1) Maximum light intensity card sleeve type circulation pool (standard type)

10mm，σv＝1.0μL， Maximum pressure 60bar (870psi), equipped with RFID tag

2) Maximum light intensity card sleeve type flow cell (high sensitivity type)

60mm，σv＝4μL， Maximum pressure 60bar (870psi), equipped with RFID tag

13. Spectral tool: data analysis software used for spectral evaluation, including spectral library and peak purity analysis functions

14. Analog output: Recorder/integrator: 100mV or 1V, output range 0.001-2AU, 1 signal output

15. Communication:

·Controller Area Network (CAN),

·RS232C, APG remote control: ready

·Start, stop, and stop signals, LAN

16. GLP characteristics:

·RFID is used for electronic recording of the status of flow cells and UV lamps (optical path length, volume, product number, serial number, test pass, usage status)

·Early Maintenance Feedback (EMF) continuously records instrument usage, such as lamp usage time and user set deadlines, and provides feedback information. Electronic record maintenance and error messages. Verify the wavelength accuracy of deuterium lamps

17. Safety and Maintenance: Comprehensive diagnosis, error detection, and display are carried out through handheld controllers and Agilent online laboratory consultants, including leak detection, safe leak handling, and outputting leak signals to shut down the pump system. Most maintenance areas have low voltage

18. Other: Electronic Temperature Control (ETC), used for the entire optical component

Performance indicators: 1260InfinityVL diode array detector (G1315D)

1. Detector type: 1024 diode elements

2. Light source: deuterium lamp and tungsten lamp

3. Number of signals: 8

4. Maximum sampling rate: 20Hz

6. Short term noise: at a wavelength of 254/4nm,<± 0.6x10-5AU; At a wavelength of 750nm, TC2 seconds

7. Drift:<0.9x10-3AU/hour at 254nm

8. Linear: at 265nm,>2.0AU (5%)

10. Wavelength range: 190~950nm

11. Wavelength accuracy: ± 1nm, automatic calibration of deuterium line lamp, verified with holmium oxide filter

12. Slot width: programmable: 1,2,4,8,16nm

13. Diode width:<1nm

14. Wavelength focusing: programmable, 1-400nm, increments of 1nm

15. Flow pool:

·Standard type: volume 13 μ L, optical path 10mm, maximum pressure resistance 120bar (1740psi)